Glycine-extended gastrin enhances somatostatin release from cultured rabbit fundic D-cells [v1; ref status: indexed, http://f1000r.es/8n]

Glycine-extended gastrin enhances somatostatin release from cultured rabbit fundic D-cells [v1; ref status: indexed, http://f1000r.es/8n]

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The role of the peptide hormone gastrin in stimulating gastric acid secretion is well established.Mature amidated gastrin is processed 8 Channel LOC from larger peptide precursor forms.Increasingly these processing intermediates, such as glycine-extended gastrin (G-Gly) and progastrin, have been shown to have biological activities of their own, often separate and complementary to gastrin.

Although G-Gly is synthesized and secreted by gastric antral G-cells, the physiological functions of this putative mediator are unclear.Gastrin and cholecystokinin (CCK) stimulate the secretion of somatostatin from gastric D-cells as part of the feedback control of gastric acid.In this study the effect of G-Gly and gastrin on the release of somatostatin from rabbit fundic D-cells was examined.

D-cells were obtained by collagenase-EDTA digestion and elutriation and cultured for 48 hours.With a 2 hour exposure to the peptides, gastrin but not G-Gly stimulated somatostatin release.Treatment of D-cells for 24 hours with gastrin or G-Gly individually, significantly enhanced subsequent basal as well as CCK- and Ball - Glove Softball Fielding - Senior GLP-1-stimulated somatostatin release.

Twenty four hours exposure to gastrin combined with G-Gly synergistically enhanced basal and agonist-stimulated somatostatin release and cellular somatostatin content.Gastrin and G-Gly may be important in the longer term regulation of D-cell function.